DETECTOR SPECIFICATIONS

Raven's Methanol Sensor overcomes these limitations, to give accurate monitoring and control of methanol concentration in sulctures of Mut⁺, Mut^- and Mut^S recombinant Pichia pastoris clones.

The system has been designed to easily connect to your existing system without using a large amount of valuable benchspace. This is a complete stand alone unit providing monitoring and control of methanol concentration, and a computer is not required. Data logging can be performed by a simple by simple connection of a 0-10 volt chart recorder to the rear panel or logged into a simple terminal program on a PC.

Although the Methanol Monitoring and Control Sensor has been designed for the control of methanol concentration in Pichia pastoris fermentation, since measurement of vapor occurs in the liquid combustible phase, this system may prove itself useful in many other applications.

Repressive carbon sources such as glucose or ethanol both exhibit different effects, and complete utilization of such carbon sources in the batch phase allows depression. Since the Raven Methanol Monitoring and Control System also detects ethanol, if during the batch phase an increase in sensor signal is observed, this may indicate ethanol production. By allowing complete exhaustion of the ethanol prior to methanol induction, repression can be avoided.

Mixed feeding protocols, mainly implement with Mut^S strains can be a very effective means of increasing productivity of your protein of interest. In the past, various compositions of mixed carbon feeds i.e. glycerol/methanol have been employed to increase productivity. With the ability to monitor and control the methanol concentration, one can separate feeding systems for glycerol addition, thus allowing the user more flexibility in maintaining limited glycerol concentrations.