Temperature:
Temperature set point = 30C +/- C.
Cooling:
Pichia pastoris fermentations generate appreciable amounts of heat during the course of fermentation. Fermentors fitted with a simple cooling finger may not offer sufficient cooling capacity, therefore a jacketed fermentor or a cooling coil fitted into the fermentor must be used to increase the surface area thereby offering an increase in cooling capacity.
Water temperature in warmer climates may not be cold enough for sufficient cooling, in which case a cooling recirculating water bath is required to maintain temperature.
Heating: This is generally not a problem. The heating systems supplied with fermentors are usually adequate. |
