Oxygen Supplementation:

After maximum agitator rpm (1,000) has been reach, supplementation oxygen is required to maintain the dissolved oxygen set point. This can be accomplished by the use of a solenoid or mass flow controller, which is activated by the fermentor dissolved oxygen .

Initial oxygen sparge rate = 0.2 to 05. vvm.

CAUTION! Oxygen is hazardous, therefore consult your gas supplier for proper guidelines.