Agitation:

• Agitator rmp = 300 min to 1000 max
• Initial air sparge rate = 1 vvm
• Agitation will ramp from the minimum to themaximum rpm value as required to maintain the dissolved oxygen set point
• Rushton type impellers (minimum 2) should be positioned to give the maximum oxygen transfer rate (OTR) - contact us if you need help with this
• The upper impeller should be positioned slightly below the initial media level to avoid excess splashing during the feeding phase as fermentor volume increases. If this is not possible due to poor OTR, the upper impeller should be positioned such that splashing does not occur near the top of the fermentor.